The efficacy of Aeromonas hydrophila GPl-04 feed-based vaccine on African catfish (Clarias gariepinus)

Abstract. Mulia DS, Utomo T, Isnansetyo A. 2022. The efficacy of Aeromonas hydrophila GPl-04 feed-based vaccine on African catfish (Clarias gariepinus). Biodiversitas 23: 1505-1510. is a pathogenic bacterium to Vaccination one the strategic efforts control this type bacterial attack. objective study was determine effectiveness for catfish. This used strain isolate as material. Sample measuring 10-13 cm length, weighing 16-25 g, taken from aquaculture ponds in Purbalingga area, Central Java, Indonesia. applied experiments under completely randomized design (CRD) method with 5 treatments and 3 replications. consisted P0: feed without (control); P1: 10 mL/100 g dose given days; P2: 15 P3: mL/ 100 P4: days. challenge test carried out week 3, by injecting 0.1 mL suspension at 105CFU/mL per fish. main parameters included fish antibody titer, survival rate (SR), relative percent (RPS), mean time death (MTD), growth rate. Supporting water quality such temperature, pH, dissolved O2 levels. parameter data were analyzed using analysis variance (ANOVA) Duncan multiple range (DMRT) 5% level, supporting descriptively quantitative analyzed. results showed could increase SR, RPS, MTD (P<0.05). In addition, vaccination did not adversely affect growth. effective protecting attack where dose, days (P1) indicated most compared other treatments.